UTSA Stem Cell Core

 

Overview of Services

The Stem Cell Core offers five basic services that function independently but can also be combined for larger projects.

  •  Isolation and Cryopreservation of Cells from Adult Tissues: The Core isolates fibroblasts, PBMCs or other cell types from donor samples and cryopreserves these for future use.
  • Reprogramming of Adult Tissues to Induced Pluripotent Stem Cells: The Core receives samples from investigators and reprograms these using non-integrating vector systems resulting in footprint free iPSCs. These samples can be any cell type and we have successfully reprogrammed cells from multiple species including humans, non-human primates and mice. Additionally, we provide a full suite of quality control options to ensure that the iPSCs produced are of the highest quality.
  • Gene Editing of Pluripotent Stem Cells: The Core designs and carries out gene editing of pluripotent stem cells using CRISPR/CAS9. These services include, in silico design of guide RNAs and homologous damage repair templates, validation of enzymatic cutting efficiencies, transfection of reagents into the cells, clonal selection of modified cells and quality control for maintenance of the pluripotent phenotype.
  • Custom Research Utilizing Pluripotent Stem Cells and their Differentiated Progeny: The Core designs and carries out custom research for investigators who do not have the experience or the infrastructure necessary to complete these experiments. These involve production of 2D and 3D differentiated cells or testing of small molecules and other reagents for biological effect or toxicity.
  • Consultation and Training: The Core provides consultation on any area of pluripotent stem cell research and training in all technologies described above.

Location and Hours of Operation

Hours Location

Monday to Friday, 9:00 a.m. – 5:00 p.m.

The University of Texas at San Antonio
One UTSA Circle
Science and Engineering Building Rm 4.166
San Antonio, TX 78249

Core Acknowledgment Policies

If you obtain data for your manuscript from use of or with the Stem Cell Core, please use the following examples to help format your acknowledgment. 

"The authors acknowledge the University of Texas at San Antonio Stem Cell Core for support during this work."

"The authors would like to thank Dr. Christopher Navara (Stem Cell Core, University of Texas at San Antonio) for technical support."

New User Access 

UTSA Users

Principal Investigators (PIs)

1) Select "sign-up" or "login" button at the top-right of this screen and register using your myUTSA ID (abc123) credentials.
2) If you are a PI with a valid Project ID assigned to you in PeopleSoft, your Group/Lab account will automatically be created with access to those avaialable funding accounts upon first-time login. Fund information is automatically imported nightly. Please contact iLab@utsa.edu if your funding information is not available in our system.

Non-PI Users

1) Select "sign-up" or "login" button at the top-right of this screen and register using your myUTSA ID (abc123) credentials.
2) Fill out the iLab registration page, select your PI Group/Lab, and verify your contact information.
3) Once your registration is submitted, the PI and/or Lab Manager will receive a notification that you have requested a membership to the Group/Lab. They will need to 1) approve your membership and 2) assign funds to your account (Speedtypes=Project ID or Cost Center) before you can submit a request and use core facility resources.

Non-UTSA Users

1) Complete the External User Access Request Form (here).

2) UTSA Staff will validate/coordinate if your institution has an active Research Service Agreement with UTSA. **This agreement must be signed by both institutions before any work can be performed. 
3) Complete the iLab registration wizard for "Non-UTSA Users".

Registration for Non-UTSA Customers
1) Select "sign-up" button at the top-right of the screen (or here
2) Receive a welcome email from iLab (typically within one business day) with login credentials.
3) Use login credentials provided to finish account creation.

4) If/once an active Service Agreement is signed, your user account will be granted access to UTSA's iLab System. 

For further information regarding this service agreement, please contact ORIS@utsa.edu.

For any assistance or general questions regarding iLab, please contact iLab@utsa.edu

Contacts

Name Role Phone Email Location
Christopher Navara
Scientific Director
 
(210) 458-6497
 
Chistopher.Navara@utsa.edu
 

 
Li-Hua Yen
Core Staff
 

 
Li-Hua.Yen@utsa.edu
 

 
Isamar Santana Toro
Core Staff
 

 
isamar.santanatoro@utsa.edu
 

 

Service Item List


Search available services: View: by category alphabetically
Fibroblast Isolation (2)
PBMC Isolation (4)
Quality Control (QC) (10)
Reprogramming (3)
Gene Editing (4)
Consumables (1)
Miscellaneous (2)

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